KFC/SSM Seminar in Spectroscopic Method

Seminar lecturer: Martin Hof
Seminar: 2 hours/week
Credits: 1
Winter semester
Form of course completion: course credit

• Electromagnetic spectrum – basic notions and quantities, calculations
• Electron absorption – transmittance, absorbance, Lambert-Beer law, extinction molar coefficient, applications, influence of pH, effect of conjugation
• Polarization of light, optical activity of species, examples of circular dichroism and optical rotational dispersion applications
• Molecular symmetry – elements of symmetry, point groups, determination of point groups, character tables, selection rules of transitions
• Vibrational spectroscopy:
  IR (infrared) spectroscopy – theory and reality of molecular vibrations, fundamental vibrations of N-atom molecules, characteristic wavenumbers of vibrations of several functional groups, experimental setup, exploitation of vibrational absorption
  Raman spectroscopy (RS) – comparison with IR, selection rules, principle, Stokes and anti-Stokes, exploitation
  Resonance Raman spectroscopy (RRS) – principle and application
  Surface-enhanced Raman spectroscopy (SERS) – principle, nanoparticles of noble metals, detection on single-molecule level, applications
  Others sophisticated techniques of RS/SERS: micro-Raman, NSOM, TERS
• Emission:
  Jablonski diagram, fluorescence – repetition of basic rules, quantum yield, lifetime, fluorescence markers and their exploitation, fluorescence microscopy, fluorescence resonance energy transfer (FRET)
• Repetition of NMR basics
• Basics of Moessbauer spectroscopy