AFC/SAB Biomacromolecules Structural Analysis
Subject title: Biomacromolecules Structural Analysis
Abbreviation: AFC/SAB
Semester taught: Summer semester
Lecturer: Assoc. prof. Michal Čajan, Ph.D.
Requirements on students: At least 60% successfulness in final written colloquium.
Course objectives: Lecture "Biomacromolecules Structural Analysis" is focused on common approaches for biomacromolecular structure analysis.
Content:
- chemical bond and its properties
- noncovalent interactions – van der Waals interaction, hydrogen bond
- molecular structure – constitution, configuration, conformation
- proteins, nucleic acids, saccharides, lipides – composition and basic structural features
- proteins, nucleic acids, saccharides, lipides – occurence and role in organisms
- factors determining 3D geometry of biomacromolecules – structure and properties relationship
- separation and isolation of biomacromolecules
- crystallography of biomacromolecules
- NMR spectrosopy of biomacromolecules
- mass spectrometry of biomacromolecules
- theoretical methods in bimolecular structure study
- basic principles of protein engineering
Literature:
- B. Rupp, Biomolecular crystallography: principles, practice, and application to structural biology, N.Y: Garland Science, New York, 2010.
- J. N. S. Evans, Biomolecular NMR spectroscopy, Oxford University Press, Oxford 1995.
- M. S. Lipton, L. Paša-Tolić, Mass spectrometry of proteins and peptides: methods and protocols. Humana Press, New York, 2009.
- M. Chance, Mass spectrometry analysis for protein-protein interactions and dynamics, Wiley, 2008.
- A. R. Leach, Molecular Modelling – Principles and Applications (2 edition), Pearson Education, 2001.
- D. Sheehan, Physical biochemistry: principles and applications, John Wiley and Sons, Chichester, 2000.
- I. Tinoco, K. Sauer, J. C. Wang, Physical chemistry: principles and applications in biological sciences, Englewood Cliffs: Prentice-Hall, 1985. ISBN 0136662803. K. H.
