CRH/MSR Molecular Plant Breeding
Lecturer: Pavel Křenek
Lecture: 2 hours/week + seminar 1 hour/week
Credits: 4
Summer semester
Form of course completion: course credit, exam
- Introduction, limitations of classical plant breeding.
- Traditional and contemporary approaches in plant genotyping and phenotyping.
- QTL mapping, linkage disequilibrium and association mapping, recombinant inbred lines, new mapping populations.
- Marker-assisted selection, genome-assisted selection and examples.
- Isolation and functional analysis of genes, in silico gene prediction, gene cloning based on cDNA sequencing, positional gene cloning.
- In vivo mutagenesis techniques, tilling, transposons. Use of mutagenesis in plant breeding.
- Targeted genome engineering of model plants and crops. Disruption, addition, substitution and precise editing of genes. Structural changes of chromosomes. Meganucleases.
- Zinc finger proteins, zinc finger nucleases and examples of successful application.
- TAL effectors, TAL effector nucleases and examples of successful application. Nanotechnology and genome engineering.
- Targeted plant transformation. Recombinase systems: CRE / lox, FLP / FRT and R / RS. Site-specific integration of a transgene. Recombinase-mediated cassette exchange. Examples of successful application.
- Methods to produce marker-free and marker-free targeted transgenic plants. Cisgenesis. Examples of successful application.
- Application of bioinformatics tools in molecular plant breeding.
During the seminar, students will present "case study" presentations related to the topic of actual lecture. Based on the "cases" presented, students are further actively discussing the topic of a lecture from a practical point of view.
