CRH/GFP Theoretical and Practical Plant GFP Technologies and Confocal Laser Microscopy

Guarantor: Jozef Šamaj
Tutorial: 2 weeks/semester
Credits: 4
Winter semester
Form of course completion: course credit

This course is an innovative combination of theoretical lectures and student seminars ("journal club"), which are closely linked to practical experiments on pre-selected student mini-projects.

First week:

• opening lectures about GFP technology and microscopy
• preparation of plant material
• transient transformation of Nicotiana benthamiana
• wholemount immunolocalization of microtubules

Second week:

• student seminar (journal club)
• microscopic in vivo observation of actin, microtubules and endosomes in stable transformed Arabidopsis plants using GFP technology
• image analysis of the results from microscopic observations
• results evaluation
• presentation of the results by the students